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Sleep & Relaxation

DSIP (Delta Sleep-Inducing Peptide)

Formula: C35H48N10O15Sequence: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu

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Written by Peptok Research
Reviewed by Medical Advisory BoardLast updated: Jan 2026

Quick Stats

Evidence Strength2/10 (Low)

Based on number and quality of indexed studies

Community Popularity1/10 (Low)

Based on search volume and community interest

Legal Status

⚖️ Not FDA-approved

Type

Sleep & Relaxation

Route

Subcutaneous injection, Intravenous, Intranasal

Half-life

~7-8 minutes (very short; effects outlast plasma levels)

Medical Disclaimer

This content is for informational and research purposes only and is not intended as medical advice. Always consult with a qualified healthcare professional before making decisions about peptide use or any medical treatment. Individual results may vary.

Overview

DSIP (Delta Sleep-Inducing Peptide) is a neuropeptide primarily known for its potential to promote sleep and reduce stress. Originally isolated from rabbit brains in the 1970s, DSIP may influence sleep architecture by modulating neurotransmitter release. Human studies suggest it has some benefits, but more research is needed.

Quick Summary

  • 🧬
    What it is:DSIP (Delta Sleep-Inducing Peptide) is a neuropeptide primarily known for its potential to promote sleep and reduce stress.
  • 🎯
    Primary use:Sleep & Relaxation applications — see benefits section for details.
  • 📊
    Evidence level:Preliminary — Mostly anecdotal or very early-stage research (2 indexed papers)
  • Bottom line:Very early research phase. Approach with appropriate caution; long-term safety is unknown.

Delta Sleep-Inducing Peptide (DSIP) is a fascinating molecule because, despite its name, its exact role in sleep regulation remains somewhat of a mystery. Isolated in 1974, DSIP was initially believed to directly induce delta wave sleep. While subsequent research hasn't fully confirmed this initial hypothesis, it has uncovered a range of potential effects, including stress reduction and potential pain relief. DSIP is a peptide with a molecular weight of only 850 daltons.

DSIP's effects are thought to stem from its interaction with various brain structures and neurotransmitter systems. It's not a direct sedative in the way that some pharmaceuticals are. Instead, research indicates it works subtly to support the body's natural sleep mechanisms. It's also being investigated for potential benefits beyond sleep, potentially due to its influence on stress hormones and pain pathways.

How DSIP (Delta Sleep-Inducing Peptide) Works

The precise mechanisms of action for DSIP are still being investigated, but several potential pathways have been identified. One key area of interest is DSIP's interaction with the GABAergic system. GABA (gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the brain, meaning it helps to calm neuronal activity. DSIP appears to enhance GABAergic transmission, potentially leading to reduced anxiety and improved sleep quality.

Another proposed mechanism involves the hypothalamic-pituitary-adrenal (HPA) axis. The HPA axis is the body's central stress response system. DSIP may help to modulate the HPA axis, reducing the release of cortisol, a stress hormone. By lowering cortisol levels, DSIP might contribute to a more relaxed state conducive to sleep.

Furthermore, DSIP may influence the release of other neurotransmitters, including serotonin and dopamine. Serotonin plays a role in mood regulation and sleep, while dopamine is involved in motivation and reward. By modulating these neurotransmitter systems, DSIP could exert a broader range of effects on mood, behavior, and sleep. Studies have also shown that DSIP may have an affinity to opioid receptors.

What the Research Actually Shows

Sleep Improvement: Human studies on DSIP and sleep have yielded mixed results. Some early research suggested that DSIP could increase slow-wave sleep (delta sleep), which is the deepest and most restorative stage of sleep. However, other studies have not consistently replicated these findings. The European Journal of Anaesthesiology published a review in 2001 summarizing the existing research on DSIP, concluding that while some studies showed positive effects on sleep, the evidence was not conclusive due to methodological limitations and small sample sizes (Pollard BJ, Pomfrett CJ. 2001). Evidence Grade: Preliminary.

Stress Reduction: There is some evidence to suggest that DSIP may have an anti-stress effect. This is thought to be related to its ability to modulate the HPA axis and reduce cortisol levels. However, most of the research in this area has been conducted in animal models. Evidence Grade: Animal only.

Pain Relief (Antinociception): Animal studies have demonstrated that DSIP can exert a potent antinociceptive (pain-relieving) effect when administered centrally (Nakamura A et al. 1988). This effect appears to be mediated by the opioid system. However, it's important to note that these findings have not been consistently replicated in human studies, and the route of administration (central administration) used in these animal studies is not typically used in humans. Evidence Grade: Animal only.

Stroke Recovery: A study published in Molecules in 2021 investigated the effects of DSIP on motor function recovery in rats after focal stroke (Tukhovskaya EA et al. 2021). The results showed that DSIP treatment led to improved motor function in the rats. This suggests that DSIP may have neuroprotective or regenerative properties. However, this is just one animal study, and further research is needed to confirm these findings and determine if they translate to humans. Evidence Grade: Animal only.

DSIP (Delta Sleep-Inducing Peptide) vs. Melatonin

Both DSIP and melatonin are associated with sleep regulation, but they work through different mechanisms. Melatonin is a hormone produced by the pineal gland that primarily regulates the sleep-wake cycle (circadian rhythm). It signals to the body that it's time to sleep, making it effective for addressing jet lag or shift work-related sleep disturbances.

DSIP, on the other hand, appears to have a more indirect effect on sleep. It may work by modulating neurotransmitter systems and reducing stress levels, creating a more conducive environment for sleep. While melatonin primarily acts as a "timekeeper," DSIP seems to function more as a "sleep enhancer." Melatonin is readily available over the counter and has a more established safety profile, while DSIP is less widely researched and its long-term effects are not fully understood.

Because they work via different mechanisms, some people find that stacking them is helpful. Melatonin can help you fall asleep, while DSIP can help improve sleep quality.

The Honest Limitations

One of the main limitations of DSIP research is the lack of large-scale, well-controlled human trials. Many of the studies conducted to date have been small, with methodological limitations that make it difficult to draw firm conclusions. Additionally, much of the research on DSIP has been conducted in animal models, and it's not always clear whether the findings will translate to humans.

Another limitation is that the precise mechanisms of action for DSIP are still not fully understood. While several potential pathways have been identified, more research is needed to elucidate how DSIP interacts with the brain and body to produce its effects. Furthermore, the optimal dosage and administration route for DSIP are not yet established. The long-term effects of DSIP use are also unknown. Kovalzon VM, Strekalova TV noted in the Journal of Neurochemistry that DSIP is a "still unresolved riddle."

DSIP Storage for Potency

DSIP is a relatively stable peptide, but proper storage is crucial to maintain its potency. Always store DSIP in a lyophilized (freeze-dried) state until you are ready to reconstitute it. Keep the lyophilized peptide in a cool, dark, and dry place, ideally in the refrigerator at 2-8°C (36-46°F). Once reconstituted with bacteriostatic water, store the solution in the refrigerator and use it within a few weeks. Avoid freezing the reconstituted solution, as this can damage the peptide structure.

Benefits & Evidence

Improved deep sleep (delta wave) quality

Moderate Evidence

2 studies · 2 human trials

Stress response modulation

Moderate Evidence

1 studies · 1 human trial

Pain reduction

Preliminary

1 studies · 0 human trials

Normalized sleep patterns

Preliminary

1 studies · 0 human trials

Reduced cortisol levels

Preliminary

1 studies · 0 human trials

LH release stimulation

Preliminary

1 studies · 0 human trials

Who Uses DSIP (Delta Sleep-Inducing Peptide)?

Research enthusiasts

Preliminary

Emerging therapeutic applications being studied

Biohackers

Anecdotal

Exploring optimization potential

Not recommended if:

Pregnant or nursing, history of hormone-sensitive cancers, active autoimmune conditions, or pediatric patients. Always consult a physician before starting any peptide protocol.

Dosage Guide

Protocol by Experience Level

ExperienceDoseFrequencyCycleRoute
Beginner100 mcgDaily or EOD4–6 wks, 2 wks offSubQ injection
Intermediate200 mcgDaily4–6 wks, 2 wks offSubQ injection
Advanced300 mcgDaily (split dose)4–6 wks, 2 wks offSubQ injection

Standard Protocol

100-300 mcg before bedtime

Notes

Despite very short half-life, effects persist for hours — suggesting it triggers signaling cascades rather than acting directly. Works best for normalizing disrupted sleep rather than forcing sleep in healthy individuals.

Route

Subcutaneous injection, Intravenous, Intranasal

Half-life

~7-8 minutes (very short; effects outlast plasma levels)

Molecular Weight

848.81 g/mol

Disclaimer

This information is for educational purposes only. Dosage information is derived from research literature and community reports. Always consult a qualified healthcare provider before using any peptide.

What the Community Reports

Community data coming soon

We're aggregating Reddit discussions for DSIP (Delta Sleep-Inducing Peptide).

Safety Profile

Regulatory Status

Not FDA-approved. Research chemical. Available through some compounding pharmacies.

Common

  • Daytime drowsiness (if dosing wrong)
  • Headache
  • Nasal irritation (intranasal)

Rare

  • Limited long-term safety data

Serious

No serious adverse events reported in available literature.

Pregnancy: ❌ Not recommended — no safety dataKnown Interactions: 3 documented stacks
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Research

Mechanism of Action

DSIP modulates multiple neurotransmitter systems. It acts on GABA-A receptors to promote sedation, reduces CRH (corticotropin-releasing hormone) to lower stress/cortisol, and modulates serotonin and glutamate signaling. It normalizes sleep architecture rather than simply inducing sedation. It also stimulates LH release and has opioidergic pain-modulating effects. The long-lasting effects despite short half-life suggest it initiates signaling cascades.

Search Volume Trend

Rank #24
12 months agoPresent
Review1990

Delta sleep-inducing peptide (DSIP): an update

Neuroscience & Biobehavioral Reviews · Graf MV, Kastin AJ

Clinical Trial1986

DSIP effects on sleep and stress in clinical trials

European Neurology · Schneider-Helmert D, Schoenenberger GA

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